SRAP markers divided the studied isolates into two distinct groups, which correlated with geographical location rather than host source. cassiicola genetic groups that infect rubber trees in Vietnam. rDNA-ITS sequence analysis revealed a new genetic group, signifying at least three C. Bootstrap values for groups one and two were 61% and 100%, respectively and the grouping was reliable, with similarity coefficient between the two main groups at 67%. cassiicola isolates belonging to cluster 2 with subgroups 2A and 2B consisted of 20 and 2 fungal isolates, respectively. Cluster one included 54 fungal isolates of which 51 and 3 were observed in subgroup 1A and 1B, respectively. cassiicola isolates into 2 main clusters. A dendrogram produced from UPGMA analysis based on Nei and Li’s coefficient divided the 76 C. All isolates could be uniquely distinguished by 208 polymorphism bands which generated 93.3% polymorphic ratio with SRAP markers. Results of rDNA-ITS sequence analysis indicated 2 nucleotide polymorphisms at the 135th (cytosine/thymine) and 474th (guanine/adenine) base pair positions of rDNA-ITS region, which differentiated the 76 studied isolates into 3 clusters. A total of 76 Corynespora cassiicola isolates collected from 16 rubber clones in several geographic regions of Vietnam, were studied for genetic diversity by ribosomal DNA internal transcribed spacer (rDNA-ITS) sequences and sequence-related amplified polymorphism (SRAP) techniques.